Study Data
| Project uploaded by: | Rajib |
| Project ID: | IMP_100022 |
| Title: | Metabolite profiling of MNSM by LC-MS/MS |
| Project Description: | LC-MS/MS analysis of Methanol extract of Mahonia napaulensis DC. Stem (MNSM) |
| Research Area: | Biological Sciences |
| Funding Source: | ICGEB, New Delhi |
| Project Contributors: | ICGEB, New Delhi and UGC, Nepal |
| Study uploaded by: | Rajib |
| Study ID: | IMS_100017 |
| Title: | Metabolite profiling of MNSM by LC-MS/MS |
| Summary: | The metabolite profiling of methanol extract of bark of M. napaulensis (MNBM) was done by LC-MS/MS analysis. The plant stem was cleaned and shade dried. The dried plant stem was ground into fine powder. The extraction was done by cold maceration process. The extract was filtered using Whatman filter paper No. 1. and was dried under reduced pressure at room temperature below 40oC, the extract was stored in closed vials at 4oC for further analysis. The metabolite profiling of methanol extract of M. napaulensis stem (MNSM) was done by LC-MS/MS analysis. The LC-MS/MS analysis identified 115 metabolites. The most abundant five metabolites are - dihydroberberine (86.05%), D-(+)-malic acid (3.43%), D-(-)-quinic acid (2.71%),(2R)-3-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-{[10-(2-hexylcyclopropyl)decanoyl] oxy}propyl palmitate (2.11%) and D-(−)-erythrose (0.83%). The literature study through PubChem and Chemical Entities of Biological Interest (ChEBI) reported that the metabolites dihydroberberine (86.05%), curcumin (0.49%), protopine (0.40%), hymecromone (0.07%), a-linolenic Acid (0.06%), menadione (0.06%), 6-gingerol (0.04%), (S)-equol (0.03%), pterostilbene (0.004%), phloretin (0.002%), 1-O-feruloyl-β-D-glucose (0.002%), and naringin (0.002%) have anticancer activity. |
| Publication: | |
| Release Date: | Feb. 13, 2025 |
| Study Type: | Mass Spectrometry (MS) |
| Data Type: | Untargeted |
| IEC/IBSC Approval Number : |
| Sr.No | Sample ID | Sample Name | Organism | Source | Sample Preparation Protocol | Sample Type | Experimental Condition | Time of treatment | Variant/Variety | Gender | Age | Replicates | Storage Conditions | Extraction Protocol |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | IMSM_100995 | M. napaulensis Stem | Mahonia napaulensis | NCBI_Taxon_ID | Concentrated metabolites were redissolved in 1 mL of 80% methanol. A 100 µL aliquot was further diluted to 5 mL with 80% methanol containing 2% formic acid. The metabolites were filtered using a 0.2 µm filter, and 10 µL was injected into the machine. | Plant Sample | Methanol Extract of stem of M. napaulensis | NA | M. napaulensis | NA | NA | NA | 4oC |
The plant stem was cleaned and shade dried. The dried plant stem was ground into fine powder. The extraction was done by cold maceration process using methanol solvent in closed vessel for three days at room temperature. The extract was collected. This process was repeated three times consecutively for the same plant sample. The extract was filtered using Whatman filter paper No. 1. The filtered extract was dried under reduced pressure at room temperature below 40oC using rotary evaporator (BIOBASE RE-2000B Rotary Evaporator, China) and then, by using freeze dryer or lyophilizer. Then, the extract was stored in closed vials at 4oC for further analysis. |
| 2 | IMSM_100996 | M. napaulensis Stem | Mahonia napaulensis | NCBI_Taxon_ID | Concentrated metabolites were redissolved in 1 mL of 80% methanol. A 100 µL aliquot was further diluted to 5 mL with 80% methanol containing 2% formic acid. The metabolites were filtered using a 0.2 µm filter, and 10 µL was injected into the machine. | Plant Sample(02) | Methanol Extract of stem of M. napaulensis | NA | M. napaulensis | NA | NA | NA | 4oC |
The plant stem was cleaned and shade dried. The dried plant stem was ground into fine powder. The extraction was done by cold maceration process using methanol solvent in closed vessel for three days at room temperature. The extract was collected. This process was repeated three times consecutively for the same plant sample. The extract was filtered using Whatman filter paper No. 1. The filtered extract was dried under reduced pressure at room temperature below 40oC using rotary evaporator (BIOBASE RE-2000B Rotary Evaporator, China) and then, by using freeze dryer or lyophilizer. Then, the extract was stored in closed vials at 4oC for further analysis. |
| Sr.No | MS Exp ID | Sample Name/ID | Mass Spectrometer Type | MS Instrument Name | MS Instrument type | MS Ionization Method | Ion Mode/Scan Polarity | Data Transformation (Software/s Used) |
|---|---|---|---|---|---|---|---|---|
| 1 | IME_100504 | M. napaulensis Stem / IMSM_100996 | LCMS (Liquid Chromatography- Mass Spectrometry) | Thermo Q Exactive Orbitrap | Orbitrap | Others | Negative | Compound Discoverer |
| 2 | IME_100505 | M. napaulensis Stem / IMSM_100995 | LCMS (Liquid Chromatography- Mass Spectrometry) | Thermo Q Exactive Orbitrap | Orbitrap | Others | Positive | Compound Discoverer |
| Sr.No | First name | Last name | Organization | Designation | |
|---|---|---|---|---|---|
| 1 | Dr Rajib Kumar | Shrestha | shrestharajib2005@ku.edu.np | Dept. of Chemical Science and Engineering School of Engineering, Kathmandu University Dhulikhel, Kavre, Nepal | scientist |
| Sr.No | ftprun ID | MS Exp ID | MS Data Files |
|---|---|---|---|
| 1 | IMR_101103 | IME_100504 | M_napaulensis_stem_N.raw |
| 2 | IMR_101104 | IME_100505 | M_napaulensis_stem_P.raw |
| Sr.No | Structure | Details |
|---|