Study Data
| Project uploaded by: | Pannaga Pavan |
| Project ID: | IMP_100029 |
| Title: | Proteomics to Unveil Orchestration of Photorespiration and Central Carbon Pathway in Microchloropsis gaditana NIES 2587 |
| Project Description: | Photosynthetic organisms have evolved and adapted strategies to overcome the limiting concentrations of CO2. In this regard, the CO2-concentrating mechanism (CCM) developed by microalgae implies an efficient machinery to acquire CO2 in limiting environment. Inorganic carbon transporters channelize CO2 towards Rubisco, however, there are significant differences in the CCM of some species and it is obscurely understood. In the present study, we performed qualitative metabolomics and proteomics on Microchloropsis gaditana, under the influence of very-low CO2 (VLC; 300 ppm, or 0.03%) and high CO2 (HC; 30,000 ppm, or 3% v/v) at the time intervals of 0, 6, 12 and 24 hrs. Our results demonstrate that HC supplementation channelizes the carbon flux towards enhancing the biomass yield, increasing up to 1.7-fold. Cyclic electron flow driven (CEF) by PSI confers energy to the cells in the case of VLC in the initial acclimatization stage. Our qualitative metabolomic analyses has identified nearly 35 essential metabolites among which significant fold-change was observed as a photorespiratory by-product, glycolate, in VLC resulting in delayed growth and lower biomass. Whole cell proteomics study was performed in M. gaditana in both VLC and HC conditions and a total of 998 proteins were identified. Cells in VLC, undergoes dynamic changes to activate biophysical CCM with the help of bicarbonate transporters. In conclusion, comprehensive changes occur inside the cell that consequently mediate the assimilation and regulation of carbon metabolic loadout such that it favours fatty acid biosynthesis in HC. In conclusion, our emphasis is to delineate carbon assimilation in M. gaditana with the help of advanced multi-omics tools and provide translational approach for the enhanced production of biofuels and biorenewables. |
| Research Area: | Biological Sciences |
| Funding Source: | NIH grant, U2C- DK119886 |
| Project Contributors: | Pannaga Pavan Jutur |
| Study uploaded by: | Pannaga Pavan |
| Study ID: | IMS_100023 |
| Title: | Integration of Metabolomics and Proteomics to Unveil Orchestration of Photorespiration and Central Carbon Pathway in Microchloropsis gaditana NIES 2587 |
| Summary: | Photosynthetic organisms have evolved and adapted strategies to overcome the limiting concentrations of CO2. In this regard, the CO2-concentrating mechanism (CCM) developed by microalgae implies an efficient machinery to acquire CO2 in limiting environment. Inorganic carbon transporters channelize CO2 towards Rubisco, however, there are significant differences in the CCM of some species and it is obscurely understood. In the present study, we performed qualitative metabolomics and proteomics on Microchloropsis gaditana, under the influence of very-low CO2 (VLC; 300 ppm, or 0.03%) and high CO2 (HC; 30,000 ppm, or 3% v/v) at the time intervals of 0, 6, 12 and 24 hrs. Our results demonstrate that HC supplementation channelizes the carbon flux towards enhancing the biomass yield, increasing up to 1.7-fold. Cyclic electron flow driven (CEF) by PSI confers energy to the cells in the case of VLC in the initial acclimatization stage. Our qualitative metabolomic analyses has identified nearly 35 essential metabolites among which significant fold-change was observed as a photorespiratory by-product, glycolate, in VLC resulting in delayed growth and lower biomass. Whole cell proteomics study was performed in M. gaditana in both VLC and HC conditions and a total of 998 proteins were identified. Cells in VLC, undergoes dynamic changes to activate biophysical CCM with the help of bicarbonate transporters. In conclusion, comprehensive changes occur inside the cell that consequently mediate the assimilation and regulation of carbon metabolic loadout such that it favours fatty acid biosynthesis in HC. In conclusion, our emphasis is to delineate carbon assimilation in M. gaditana with the help of advanced multi-omics tools and provide translational approach for the enhanced production of biofuels and biorenewables. |
| Publication: | |
| Release Date: | March 21, 2025 |
| Study Type: | Mass Spectrometry (MS) |
| Data Type: | Untargeted |
| IEC/IBSC Approval Number : |
| Sr.No | Sample ID | Sample Name | Organism | Source | Sample Preparation Protocol | Sample Type | Experimental Condition | Time of treatment | Variant/Variety | Gender | Age | Replicates | Storage Conditions | Extraction Protocol |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | IMSM_101080 | HC_12hr_1 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | high CO2 (30,000 ppm) | 12hr | NA | NA | NA | NA | NA |
Sonication |
| 2 | IMSM_101081 | HC_12hr_2 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | high CO2 (30,000 ppm) | 12hr | NA | NA | NA | NA | NA |
Sonication |
| 3 | IMSM_101082 | HC_12hr_3 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | high CO2 (30,000 ppm) | 12hr | NA | NA | NA | NA | NA |
Sonication |
| 4 | IMSM_101083 | HC_24hr_1 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | high CO2 (30,000 ppm) | 24hr | NA | NA | NA | NA | NA |
Sonication |
| 5 | IMSM_101084 | HC_24hr_2 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | high CO2 (30,000 ppm) | 24hr | NA | NA | NA | NA | NA |
Sonication |
| 6 | IMSM_101085 | HC_24hr_3 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | high CO2 (30,000 ppm) | 24hr | NA | NA | NA | NA | NA |
Sonication |
| 7 | IMSM_101086 | HC_6hr_1 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | high CO2 (30,000 ppm) | 6hr | NA | NA | NA | NA | NA |
Sonication |
| 8 | IMSM_101087 | HC_6hr_2 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | high CO2 (30,000 ppm) | 6hr | NA | NA | NA | NA | NA |
Sonication |
| 9 | IMSM_101088 | HC_6hr_3 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | high CO2 (30,000 ppm) | 6hr | NA | NA | NA | NA | NA |
Sonication |
| 10 | IMSM_101089 | VLC_12hr_1 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | very-low CO2 (300 ppm) | 12hr | NA | NA | NA | NA | NA |
Sonication |
| 11 | IMSM_101090 | VLC_12hr_2 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | very-low CO2 (300 ppm) | 12hr | NA | NA | NA | NA | NA |
Sonication |
| 12 | IMSM_101091 | VLC_12hr_3 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | very-low CO2 (300 ppm) | 12hr | NA | NA | NA | NA | NA |
Sonication |
| 13 | IMSM_101092 | VLC_24hr_1 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | very-low CO2 (300 ppm) | 24hr | NA | NA | NA | NA | NA |
Sonication |
| 14 | IMSM_101093 | VLC_24hr_2 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | very-low CO2 (300 ppm) | 24hr | NA | NA | NA | NA | NA |
Sonication |
| 15 | IMSM_101094 | VLC_24hr_3 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | very-low CO2 (300 ppm) | 24hr | NA | NA | NA | NA | NA |
Sonication |
| 16 | IMSM_101095 | VLC_6hr_1 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | very-low CO2 (300 ppm) | 6hr | NA | NA | NA | NA | NA |
Sonication |
| 17 | IMSM_101096 | VLC_6hr_2 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | very-low CO2 (300 ppm) | 6hr | NA | NA | NA | NA | NA |
Sonication |
| 18 | IMSM_101097 | VLC_6hr_3 | Microchloropsis | Algae | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. | Algae | very-low CO2 (300 ppm) | 6hr | NA | NA | NA | NA | NA |
Sonication |
| Sr.No | MS Exp ID | Sample Name/ID | Mass Spectrometer Type | MS Instrument Name | MS Instrument type | MS Ionization Method | Ion Mode/Scan Polarity | Data Transformation (Software/s Used) |
|---|---|---|---|---|---|---|---|---|
| 1 | IME_100554 | HC_12hr_1 / IMSM_101080 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 2 | IME_100555 | HC_12hr_2 / IMSM_101081 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 3 | IME_100556 | HC_12hr_3 / IMSM_101082 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 4 | IME_100557 | HC_24hr_1 / IMSM_101083 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 5 | IME_100558 | HC_24hr_2 / IMSM_101084 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 6 | IME_100559 | HC_24hr_3 / IMSM_101085 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 7 | IME_100560 | HC_6hr_1 / IMSM_101086 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 8 | IME_100561 | HC_6hr_2 / IMSM_101087 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 9 | IME_100562 | HC_6hr_3 / IMSM_101088 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 10 | IME_100563 | VLC_12hr_1 / IMSM_101089 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 11 | IME_100564 | VLC_12hr_2 / IMSM_101090 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 12 | IME_100565 | VLC_12hr_3 / IMSM_101091 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 13 | IME_100566 | VLC_24hr_1 / IMSM_101092 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 14 | IME_100567 | VLC_24hr_2 / IMSM_101093 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 15 | IME_100568 | VLC_24hr_3 / IMSM_101094 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 16 | IME_100569 | VLC_6hr_1 / IMSM_101095 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 17 | IME_100570 | VLC_6hr_2 / IMSM_101096 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| 18 | IME_100571 | VLC_6hr_3 / IMSM_101097 | GCMS (Gas Chromatography -Mass Spectrometry) | Agilent 7890A | Triple quadrupole | Electron Ionization - EI | Positive | NA |
| Sr.No | First name | Last name | Organization | Designation | |
|---|---|---|---|---|---|
| 1 | Pannaga Pavan | Jutur | jppavan@icgeb.res.in | International Centre for Genetic Engineering and Biotechnology, New Delhi | principal_investigator |
| Sr.No | ftprun ID | MS Exp ID | MS Data Files |
|---|---|---|---|
| 1 | IMR_101153 | IME_100554 | HC_12hr_1.mzdata.xml |
| 2 | IMR_101154 | IME_100555 | HC_12hr_2.mzdata.xml |
| 3 | IMR_101155 | IME_100556 | HC_12hr_3.mzdata.xml |
| 4 | IMR_101156 | IME_100557 | HC_24hr_1.mzdata.xml |
| 5 | IMR_101157 | IME_100558 | HC_24hr_2.mzdata.xml |
| 6 | IMR_101158 | IME_100559 | HC_24hr_3.mzdata.xml |
| 7 | IMR_101159 | IME_100560 | HC_6hr_1.mzdata.xml |
| 8 | IMR_101160 | IME_100561 | HC_6hr_2.mzdata.xml |
| 9 | IMR_101161 | IME_100562 | HC_6hr_3.mzdata.xml |
| 10 | IMR_101162 | IME_100563 | VLC_12hr_1.mzdata.xml |
| 11 | IMR_101163 | IME_100564 | VLC_12hr_2.mzdata.xml |
| 12 | IMR_101164 | IME_100565 | VLC_12hr_3.mzdata.xml |
| 13 | IMR_101165 | IME_100566 | VLC_24hr_1.mzdata.xml |
| 14 | IMR_101166 | IME_100567 | VLC_24hr_2.mzdata.xml |
| 15 | IMR_101167 | IME_100568 | VLC_24hr_3.mzdata.xml |
| 16 | IMR_101168 | IME_100569 | VLC_6hr_1.mzdata.xml |
| 17 | IMR_101169 | IME_100570 | VLC_6hr_2.mzdata.xml |
| 18 | IMR_101170 | IME_100571 | VLC_6hr_3.mzdata.xml |